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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Cancer Lett. 2020 Dec 21;500:220–227. doi: 10.1016/j.canlet.2020.12.023

Fig. 1.

Fig. 1.

YTR107 inhibits NPM1/RAD51 interactions. A) HEK293 cells were transfected with a plasmid that expressed a chimeric GFP/FLAG/NPM1 protein. Five days after transfection the cells were exposed to 0 or 35 μM YTR107 for 30 min prior to administering 0 or 10 Gy. Two hrs later cells were solubilized in 0.5% NP40 buffer. Protein was immunoprecipitated using a RAD51 antibody. Immunoprecipitated protein was immunoblotted with FLAG antibody. The expression of immunoprecipitated GFP/FLAG/NPM1 relative to immunoprecipitated RAD51 is shown at the top of figure. B) HEK293 cells were transfected with a plasmid that expressed a chimeric GFP/FLAG/NPM1 protein. Five days after transfection the cells were exposed to 0 or 25 μM YTR107 for 30 min prior to administering 0 or 10 Gy. Two hrs later cells were solubilized in 0.5% NP40 buffer. Protein was immunoprecipitated using a SUMO-1 antibody. Immunoprecipitated protein was immunoblotted with FLAG antibody.