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. 2020 Dec 22;9(1):7. doi: 10.3390/microorganisms9010007

Figure 3.

Figure 3

Amino acid uptake by S. cerevisiae cells. Transporters were expressed from the constitutive ADH1 promoter with a C-terminal YPet tag. BY4709 carrying the empty plasmid pRSII426 was used as a vector control strain. (A) Uptake of phenylalanine (2.5 mM). (B) Uptake of lysine (50 µM) in the absence (black symbols) or presence of histidine (100 mM, grey symbols) or ornithine (100 mM, white symbols); circles, Lyp1 overexpression; squares, vector control strain. (C) Uptake of either alanine (500 µM, black symbols) or methionine (500 µM, white symbols); diamonds, Agp1 overexpression; squares, vector control strain. The straight black lines in (B,C) represent the calculated initial uptake rates, which are shown in (D,E), respectively. Values are the mean of biological triplicates. Error bars represent standard deviation and, in some cases, are obscured by the data point. Rates in (D) were compared using a t-test, and asterisks (*) represent p < 0.001.