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. 2020 Dec 22;13(1):9. doi: 10.3390/nu13010009

Figure 1.

Figure 1

Biosynthetic forms of wild type sucrase-isomaltase (SI) (SI-WT) and SI mutants in COS-1 cells. SI-WT or SI-mutants (R250C, R1484H, E640G, F1625V, P1200S, Y867H, Y1417X and V717D) were expressed in COS-1 cells, immunoprecipitated with mAb anti-SI and treated or not treated with endo H then analyzed by Western blotting. The mature, endo H-resistant form of SI has a molecular weight of 245 kDa; the mannose-rich ER form of SI (210 kDa) is sensitive to endo H and shifts to a lower Band at 185 kDa.