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Cellular localization of wild type SI (SI-WT) and SI mutants in COS-1 cells. The SI-WT or Scheme 488. (green). Calnexin was utilized as a protein marker of the ER. Here, rabbit anti-calnexin antibody was used as a primary antibody and goat anti-rabbit IgG (secondary) carrying Alexa flour 568 (red). DAPI labels the nucleus (blue). Bars = 30µm.
