FIG 5.
Treatment with anti-Nrp-1 reduced the mRNA expression of foxp3, ctla4, and tgf-β1 in CD4+ CD25+ Tregs in a sepsis model in mice (a). Shown are statistical figures and representative immunofluorescence images of Foxp-3 (b, d) and CTLA-4 (c, d) expression at the protein level, the levels of secretion of IL-10, TGF-β1, IFN-γ, and IL-4 in serum (e), statistical figures and representative EMSA images of the DNA-binding activity of NF-κB (f, g), and the effects of anti-Nrp-1 (administered at different concentrations) on histopathological changes (magnification, ×200) in the renal tissues of mice with CLP-induced sepsis (h, i). (d, g) 1: control; 2: LPS; 3: anti-Nrp-1(0.5 mg/Kg); 4: anti-Nrp-1(2.5 mg/Kg); 5: anti-Nrp-1(5 mg/Kg). Data are presented as means ± SD (n, 4 per group). Asterisks indicate significance (*, P < 0.05; **, P < 0.01) by one-way ANOVA.