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PpnN is regulated in a (p)ppGpp-dependent manner. (A and B) Transcriptional reporter of the full-length ppnN promoter (A) and argI promoter (B), a canonical gene target regulated during the stringent response, showed (p)ppGpp-dependent up-regulation. RLU, relative light units. Data are the means ± SEM (error bars) from three independent experiments. Strains are carrying the empty pWSK129 vector control unless otherwise specified. ns, not significant; *, P < 0.05; ***, P < 0.0001.
