Figure 2.

Molecular phylogenetic analysis, based on 16S rRNA partial gene, showed the position of Methanomassiliicoccus-like sequences detected in feces of pigs. The evolutionary history was inferred using the Neighbor-Joining method. The optimal tree with the sum of branch length = 0.82722721 is shown. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1.000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the Maximum Composite Likelihood method and are in the units of the number of base substitutions per site. The analysis involved 14 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 415 positions in the final dataset. Evolutionary analyses were conducted in MEGA7. Bootstrap values ≥ 95% are indicated at nodes. In red: out of group. Species highlighted: methanogens species detected in this study.