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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Pigment Cell Melanoma Res. 2019 Oct 16;33(2):279–292. doi: 10.1111/pcmr.12828

FIGURE 6.

FIGURE 6

Mitf isoform-specific knockout mice have distinct eye phenotypes. (a) Representative images of whole eyes of indicated mice before (top) and following enucleation of iris with pupil indicated (middle) and posterior surface of the eye (bottom). (b) Melanin quantification on dissected iris of indicated mice, n = 3. (c) Pigment assay on combined RPE, choroid, and sclera of wild-type, Mitf-A−/−, Mitf-M−/−, and Albino mice, n = 4. (d) H&E sections from paraffin-embedded eyes with choroid and RPE indicated by arrows. (e) cKit staining of choroid, with RPE highlighted and cKit-positive melanocytes indicated with arrows. The inset area is highlighted by the red box. (f) Relative expression of Mitf isoforms in the isolated RPE and choroid of wild-type and Mitf isoform-specific mutant mice, n = 3. (g) Further comparison of Mitf isoform abundance in the RPE and choroid of wild-type and Mitf knockout mice