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. 2021 Jan 18;14:227–240. doi: 10.2147/DMSO.S289712

Figure 1.

Figure 1

Histopathological examination of nondiabetic adjacent normal kidney tissues (NC) and renal biopsy sections from DN patients. Representative images showing (A) Masson’s trichrome staining, (B) PAS staining, (C) immunohistochemical staining for AdipoR1, and (D) immunohistochemical staining for p-ACC. All the images are magnified 200×. Quantitative analysis of the (E) percentage of the collagen fiber area, as shown by Masson’s trichrome staining, (F) Mean basement membrane thickness of renal tubules, as shown by PAS staining, (G) Cumulative optical density values for AdipoR1 and (H) p-ACC, as determined by immunohistochemistry. All the data are expressed as means ± SEMs (*p < 0.05 vs NC).