Table 2. List of differentially accumulated proteins in Q. suber leaf proteome 8 months after P. cinnamomi inoculation, using the Arabidopis proteome database as a reference.
| Arabidopis UniProt accessiona | Median C (x10-3)b | Median I (x10-3)b | p≤0.05d | log2FCc | Protein namea | Protein Initialsa |
|---|---|---|---|---|---|---|
| Proteins more abundant in P. cinnamomi inoculated samples compared to the control | ||||||
| P27323 | 0.056 | 0.169 | 0.015 | 1.7 | Heat shock protein 90–1 | HS90-1 |
| Q9FIF3 | 0.120 | 0.274 | 0.041 | 1.4 | 40S ribosomal protein S8-2 | RS82 |
| O81644 | 0.020 | 0.037 | 0.132 | 1.3 | Villin-2 | VILI2 |
| P38418 | 0.188 | 0.529 | 0.24 | 1.2 | Lipoxygenase 2, chloroplastic | LOX2 |
| A0A1P8AWT7 | 0.533 | 1.361 | 0.041 | 1.1 | Catalase 3 | A0A1P8AWT7 |
| Q940B0 | 0.232 | 0.436 | 0.065 | 1.1 | 60S ribosomal protein L18-3 | RL183 |
| A8MRL0 | 0.183 | 0.397 | 0.015 | 1.1 | Histone superfamily protein | A8MRL0 At4G40030 |
| Q9FGX1 | 0.124 | 0.260 | 0.004 | 0.9 | ATP-citrate synthase beta chain protein 2 | ACLB2 |
| O04499 | 0.098 | 0.195 | 0.009 | 0.9 | 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 | PMG1/iPGAM |
| O49485 | 0.575 | 1.064 | 0.041 | 0.9 | D-3-phosphoglycerate dehydrogenase 1, chloroplastic | SERA1 |
| Q9LF37 | 0.038 | 0.074 | 0.041 | 0.9 | Chaperone protein ClpB3, chloroplastic | CLPB3 |
| Q9STX5 | 0.175 | 0.389 | 0.041 | 0.9 | Endoplasmin homolog | ENPL |
| Q9M040 | 0.171 | 0.336 | 0.009 | 0.8 | Pyruvate decarboxylase 4 | PDC4 |
| Q9SIH0 | 0.142 | 0.257 | 0.004 | 0.8 | 40S ribosomal protein S14-1 | RS141 |
| Q9SIM4 | 0.302 | 0.495 | 0.015 | 0.7 | 60S ribosomal protein L14-1 | RL141 |
| Q93ZN2 | 0.282 | 0.437 | 0.041 | 0.7 | Probable aldo-keto reductase 4 | ALKR4 |
| Q9LKR3 | 1.119 | 1.835 | 0.041 | 0.7 | Mediator of RNA polymerase II transcription subunit 37a | MD37A |
| Q9FMP3 | 1.183 | 2.187 | 0.026 | 0.7 | Dihydropyrimidinase | DPYS |
| Q9S9N1 | 1.651 | 2.616 | 0.004 | 0.7 | Heat shock 70 kDa protein 5 | HSP7E/BiP1 |
| P42798 | 0.320 | 0.489 | 0.004 | 0.6 | 40S ribosomal protein S15a-1 | R15A1 |
| A8MS03 | 0.126 | 0.199 | 0.026 | 0.6 | Ribosomal protein S6 | A8MS03 |
| A8MS28 | 0.481 | 0.754 | 0.026 | 0.6 | Ribosomal L27e protein family | A8MS28 |
| Q9SEI3 | 0.326 | 0.496 | 0.026 | 0.6 | 26S proteasome regulatory subunit 10B homolog A | PS10A/RTP4A |
| Q9SII0 | 0.257 | 0.395 | 0.009 | 0.6 | Probable histone H2A variant 2 | H2AV2 |
| Q39142 | 2.316 | 3.472 | 0.041 | 0.6 | Chlorophyll a-b binding protein, chloroplastic | Q39142 |
| P16181 | 0.224 | 0.332 | 0.009 | 0.5 | 40S ribosomal protein S11-1 | RS111 |
| Q9SRV5 | 2.348 | 3.690 | 0.041 | 0.5 | 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase 2 | METE2 |
| P49107 | 0.604 | 1.064 | 0.004 | 0.5 | Photosystem I reaction center subunit N, chloroplastic | PSAN |
| P59259 | 9.892 | 14.422 | 0.041 | 0.5 | Histone H4 | H4/HIS4 |
| Q9LHA8 | 0.325 | 0.467 | 0.015 | 0.5 | Probable mediator of RNA polymerase II transcription subunit 37c | MD37C |
| O04486 | 0.251 | 0.354 | 0.041 | 0.5 | Ras-related protein RABA2a | RAA2A |
| P59233 | 3.624 | 5.261 | 0.015 | 0.5 | Ubiquitin-40S ribosomal protein S27a-3 | R27AC |
| Q8W4H7 | 7.269 | 10.076 | 0.015 | 0.5 | Elongation factor 1-alpha 2 | EF1A2 |
| P52577 | 2.145 | 3.732 | 0.002 | 0.5 | Isoflavone reductase homolog P3 | IFRH |
| F4JWF7 | 0.616 | 0.849 | 0.041 | 0.5 | DEAD/DEAH box RNA helicase family protein | F4JWF7 |
| Q9SVR0 | 0.114 | 0.183 | 0.009 | 0.5 | 60S ribosomal protein L13a-3 | R13A3 |
| P59224 | 2.369 | 3.154 | 0.009 | 0.5 | 40S ribosomal protein S13-2 | RS132 |
| Q9SRZ6 | 0.376 | 0.563 | 0.026 | 0.4 | Cytosolic isocitrate dehydrogenase [NADP] | ICDHC/cICDH |
| Q9LZH9 | 0.282 | 0.384 | 0.009 | 0.4 | 60S ribosomal protein L7a-2 | RL7A2 |
| Q9LD28 | 0.620 | 1.017 | 0.041 | 0.4 | Histone H2A.6 | H2A6 |
| Q948K6 | 0.232 | 0.315 | 0.015 | 0.4 | Ras-related protein RABG1 | RABG1 |
| P22953 | 0.397 | 0.573 | 0.026 | 0.4 | Probable mediator of RNA polymerase II transcription subunit 37e | MD37E |
| Q9SU58 | 0.146 | 0.209 | 0.015 | 0.4 | ATPase 4, plasma membrane-type | PMA4 |
| Q8H156 | 1.697 | 2.498 | 0.009 | 0.4 | GTP-binding nuclear protein Ran-3 | RAN3 |
| A0A1P8B2Y6 | 0.197 | 0.287 | 0.026 | 0.4 | Plasma membrane ATPase | A0A1P8B2Y6 |
| A8MS75 | 1.813 | 2.570 | 0.026 | 0.4 | Chlorophyll a-b binding protein, chloroplastic | A8MS75 |
| Q9FJA6 | 1.027 | 1.379 | 0.004 | 0.4 | 40S ribosomal protein S3-3 | RS33 |
| Q9FF90 | 0.841 | 1.131 | 0.015 | 0.4 | 60S ribosomal protein L13-3 | RL133 |
| F4J3P1 | 1.255 | 1.589 | 0.004 | 0.4 | Ribosomal protein L14p/L23e family protein | F4J3P1 |
| Q9LXG1 | 0.243 | 0.382 | 0.026 | 0.4 | 40S ribosomal protein S9-1 | RS91 |
| Q6ICZ8 | 0.167 | 0.250 | 0.002 | 0.3 | Nascent polypeptide-associated complex subunit alpha-like protein 3 | NACA3 |
| P0CJ47 | 0.700 | 0.970 | 0.026 | 0.3 | Actin-3 | ACT3 |
| Q9SZ54 | 0.346 | 0.476 | 0.041 | 0.3 | Putative glutathione peroxidase 7, chloroplastic | GPX7 |
| A0A1P8B767 | 0.610 | 0.773 | 0.041 | 0.3 | Quinone reductase family protein | A0A1P8B767 |
| Q8LB10 | 0.117 | 0.141 | 0.015 | 0.3 | ATP-dependent Clp protease proteolytic subunit-related protein 4, chloroplastic | CLPR4 |
| F4JJ94 | 0.801 | 1.008 | 0.026 | 0.3 | General regulatory factor 1 | F4JJ94 |
| Q9LUD4 | 0.326 | 0.414 | 0.041 | 0.3 | 60S ribosomal protein L18a-3 | R18A3 |
| Q93VH9 | 2.069 | 2.492 | 0.015 | 0.2 | 40S ribosomal protein S4-1 | RS41 |
| O23254 | 0.837 | 1.142 | 0.041 | 0.2 | Serine hydroxymethyltransferase 4 | GLYC4 |
| O49299 | 2.435 | 3.204 | 0.002 | 0.2 | Probable phosphoglucomutase, cytoplasmic 1 | PGMC1/PGM1 |
| Proteins less abundant in P. cinnamomi inoculated samples compared to the control | ||||||
| F4J3Q8 | 0.345 | 0.099 | 0.004 | -3.5 | P-loop containing nucleoside triphosphate hydrolases superfamily | F4J3Q8 |
| P10795 | 0.878 | 1.543 | 0.818 | -3.1 | Ribulose bisphosphate carboxylase small chain 1A, chloroplastic | RBS1A/RBCS1A |
| Q9FLN4 | 0.218 | 0.100 | 0.132 | -1.5 | 50S ribosomal protein L27, chloroplastic | RK27 |
| Q9FZ47 | 1.255 | 0.641 | 0.065 | -1.3 | ACT domain-containing protein ACR11 | ACR11 |
| O04603 | 0.412 | 0.201 | 0.041 | -1.2 | 50S ribosomal protein L5, chloroplastic | RK5 |
| Q8RX32 | 0.513 | 0.260 | 0.026 | -0.6 | Tropinone reductase homolog At1g07450 | TRNH2 |
| Q9SCW1 | 0.184 | 0.110 | 0.026 | -0.6 | Beta-galactosidase 1 | BGAL1 |
| F4JYM8 | 0.543 | 0.294 | 0.026 | -0.6 | Thiolase family protein | F4JYM8 |
| A0A1P8B485 | 0.402 | 0.257 | 0.015 | -0.6 | Protein translocase subunit SecA | A0A1P8B485 |
| P25697 | 8.890 | 6.489 | 0.015 | -0.6 | Phosphoribulokinase, chloroplastic | KPPR/PRK |
| Q9LRR9 | 1.487 | 0.662 | 0.002 | -0.6 | (S)-2-hydroxy-acid oxidase GLO1 | GLO1/GOX1 |
| B3H4S6 | 0.434 | 0.300 | 0.041 | -0.3 | Dicarboxylate transporter 1 | B3H4S6 |
| P56778 | 19.522 | 15.325 | 0.002 | -0.3 | Photosystem II CP43 reaction center protein | PSBC |
| P56761 | 14.189 | 11.445 | 0.015 | -0.3 | Photosystem II D2 | PSBD |
| Q9LF98 | 2.338 | 1.901 | 0.041 | -0.3 | Fructose-bisphosphate aldolase 8, cytosolic | ALFC8/FBA8 |
| F4KDZ4 | 2.902 | 1.688 | 0.026 | -0.3 | Malate dehydrogenase | F4KDZ4/PMDH2 |
| Q42525 | 0.553 | 0.340 | 0.026 | -0.3 | Hexokinase-1 | HXK1 |
| P27140 | 7.947 | 6.602 | 0.041 | -0.2 | Beta carbonic anhydrase 1, chloroplastic | BCA1 |
| A0A1P8BG37 | 3.388 | 2.750 | 0.041 | -0.2 | Photosystem II stability/assembly factor, chloroplast | A0A1P8BG37 |
| Q9SAU2 | 1.863 | 1.309 | 0.026 | -0.2 | D-ribulose-5-phosphate-3-epimerase | Q9SAU2/RPE |
aUniProt accession, protein name and protein initials arise from the annotation using the Arabidopsis proteome database as a reference. For more details on the abundance levels per replicate consult S2 Table.
bData generated from SWATH-MS proteomics: median peak areas for 6 control cork oak plants (Median C) and 6 plants inoculated with P. cinnamomi (Median I).
cFold change ratio logarithm of protein abundance in inoculated over control samples greater than 1 or less than -1 (Log2FC).
dNon-parametric Mann Whitney U-test (MW) with statistical significance level set to less than 5% (p<0.05).