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. 2021 Jan 22;16(1):e0245369. doi: 10.1371/journal.pone.0245369

Fig 4. The PARP inhibitor olaparib abolishes PARP activity signals.

Fig 4

Retinal sections derived from post-natal (P) day 11 rd1 animals were incubated with NAD+ analogues, together with the selective PARP inhibitor olaparib. In both the two-step assay with 6-Biotin-17-NAD+ (A, B) and the single-step assay using 6-Fluo-10-NAD+ (C, D), olaparib, at 100 nM, completely abolished positive PARP activity detection. DAPI was used as nuclear counterstaining. ONL = outer nuclear layer, INL = inner nuclear layer, GCL = ganglion cell layer. Images representative for assays performed on tissue sections derived for at least three different rd1 animals per assay condition.