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. 2021 Jan 22;16(1):e0245614. doi: 10.1371/journal.pone.0245614

Fig 1. High specificity of secKL antibody for secKL protein.

Fig 1

(A) secKL Ab detects rsecKL protein in a direct ELISA setting over a high dilution range: 1:1000, 1:5000, 1:7000, 1:10000, 1:25000, 1:50000, 1:100000, 1:200000, 1:400000 (B) secKL Ab detects rsecKL protein in an indirect ELISA setting via HRP labelled secondary Ab (IgG dilutions: 1:1000, 1:5000, 1:10000, 1:15000, 1:20000, 1:25000, 1:30000, 1:40000, 1:50000, 1:100000) (C) secKL Ab can detect rsecKL but not rsKL protein in a direct ELISA setting at a higher dilution range (Ab dilutions: 1:1000, 1:5000, 1:7000, 1:10000, 1:15000, 1:25000, 1:50000) (D) secKL Ab can detect rsecKL but not rsKL protein in a direct ELISA setting at a lower dilution range (Ab dilutions: 1:100, 1:200, 1:400, 1:500, 1:600, 1:700, 1:800, 1:900, 1:1000) (E) Labelled detection antibody from IBL kit can detect both, rsKL and rsecKL proteins in a direct ELISA setting (Ab dilutions: 1:50, 1:100, 1:200, 1:400, 1:500, 1:700, 1:900, 1:1000) (F) secKL Ab can specifically detect rsecKL protein (6ng/ml) in the presence of indicated spiked-in non-specific proteins (3ng/ml).