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. 2021 Feb;23(2):111–118. doi: 10.1016/j.jcyt.2020.06.001

Figure 1.

Fig. 1

Induced PSCs can be generated from hWGE tissues. (A) Images of hESC and human iPSC colonies. (B) RT-PCR of OCT4, SOX2, NANOG, LIN28 and C-MYC expression in undifferentiated hWGE iPSCs. (C) ICC for OCT4 (green), SOX2 (green) and TRA-1-60 (red) in undifferentiated hWGE iPSCs. (D) ICC for endoderm (vimentin, left, red; AFP, right, red), mesoderm (α-SMA, left, red; desmin, right, green) and ectoderm (nestin, left, green; β-III tubulin, right, green) with Hoechst (blue) in in vitro spontaneously differentiated hWGE iPSCs. (E) Hematoxylin and eosin staining in teratomas from immune-deficient mice following subcutaneous injection of hWGE iPSCs. Arrows indicate adipose and gland-like tissues (endoderm), chondroblasts and smooth muscle fibers (mesoderm) and neural rosettes (ectoderm). RT-PCR, reverse transcription-polymerase chain reaction; ICC, immunocytochemistry staining; AFP, alpha fetoprotein; SMA, smooth muscle actin. (Color version of figure is available online).