| Reagents and Chemicals | Chemical Formula (If Applicable) | Quantity |
| L-sorbose | C6H12O6 | 2 g |
| L-asparagine | C4H8N2O3 | 2 g |
| Dipotassium phosphate | K2HPO4 | 1 g |
| Potassium chloride | KCl | 1 g |
| Magnesium sulfate heptahydrate | MgSO4.7H2O | 0.5 g |
| Ferric-sodium salt (FeNaEDTA) | C10H12N2O8FeNa | 0.01 g |
| Agar | 20 g | |
| Streptomycin sulphate | C42H84N14O36S3 | 0.3 g |
| Chlortetracycline hydrochloride | C22H24Cl2N2O8 | 0.05 g |
| Pentachloronitrobenzene | C6Cl5NO2 | 0.8 g |
| Borax | NaB4O7.10H2O | 1 g |
| Distilled water | 1 L |
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