| Reagents and Chemicals | Chemical Formula (If Applicable) | Quantity |
| Monopotassium phosphate | KH2PO4 | 3.0 g |
| Disodium phosphate | Na2HPO4 | 6.0 g |
| Sodium chloride | NaCl | 0.5 g |
| Ammonium chloride | NH4Cl | 1.0 g |
| Calcium chloride solution (sterilised) (0.01M) | CaCl2 | 10.0 mL |
| Magnesium sulfate heptahydrate (sterilised) (1M) | MgSO4.7H2O | 1.0 mL |
| Itaconic acid solution (filter sterilised) (20%) | C5H6O4 | 10 mL |
| Distilled water | H2O | 1 L |
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