Figure 3.

Design of the preclinical testing of MTK. Experimental setup of the pharmacoexposure and kinetics experiment in wildtype (WT) and 5xFAD mice. (A) Montelukast (MTK) films for mice were prepared as punches suitable for placing the films on the tongue of mice. (B) In the pre-experiment, C57Bl6 mice were treated with a single dose of MTK. Blood samples were taken 1, 3 and 7 h after systemic application. (C) Five-month-old 5xFAD transgenic mice were treated daily with vehicle or MTK in two different doses for 89 days. On the last day of treatment, mice were perfused and blood and cerebrospinal fluid (CSF) samples were collected approximately seven hours after the last application of MTK.