Skip to main content
. Author manuscript; available in PMC: 2021 Jan 22.
Published in final edited form as: Cytokine. 2016 Mar 28;83:53–60. doi: 10.1016/j.cyto.2016.03.015

Fig. 4.

Fig. 4.

HNP1-triggered signaling in CAL-1 cells. Serum-starved CAL-1 cells (5 × 106/ml) were treated with indicated concentrations of HNP1 for 30–60 min before they were used for the preparation of whole cell lysate or cytoplasmic/nuclear fraction. Identical amount of whole cell lysate or cytoplasmic/nuclear fraction was loaded to a SDS-PAGE gel and separated by electrophoresis. After transfer of the separated proteins onto a piece of PVDF membrane, the membrane was probed by Western blot with anti-I-κBα (A), anti-p50/anti-p65 (B), or anti-IRF1 (C). Subsequently, the membranes were stripped and re-probed with anti-GAPDH (A and left panels of B and C) or anti-lamin (right panels of B and C). Shown are the images of one experiment representative of three.