Table 3. The monoisotopic mass and relative abundance of the different OCN forms detected in mouse bone homogenates.
Monoisotopic mass range | Relative abundance (%) | Most probable modification | Most probable oligosaccharide |
---|---|---|---|
O-glycosylated OCN | 99.07 | ||
5855.6676 | 0.54 | Glycosylation + 2x Gla | HexNAc, Hex, NANA |
5899.7161 | 7.43 | Glycosylation + 3x Gla | HexNAc, Hex, NANA |
5915.6386–6135.6796 | 36.07 | Glycosylation + 3x Gla + oxidation + additional unidentified modifications or adduct ions | HexNAc, Hex, NANA |
6146.7609–6162.7991 | 5.42 | Glycosylation + 2x Gla + additional unidentified modifications | HexNAc, Hex, 2xNANA |
6190.8061 | 4.49 | Glycosylation + 3x Gla | HexNAc, Hex, 2xNANA |
6206.8016–6441.7636 | 45.12 | Glycosylation + 3x Gla + oxidation + additional unidentified modifications or adduct ions | HexNAc, Hex, 2xNANA |
Non O-glycosylated OCN | 0.93 | ||
5259.4204 | 0.93 | 3x Gla + oxidation | NA |
Gla: Gamma-carboxyglutamic acid residue; HexNAc: N-acetylhexosamine; Hex: Hexose; NANA: N-acetylneuraminic acid; 1x: one time; 2x: two times; 3x: three times; NA: not applicable.