Figure 5.

Effects of soy-based diets in the testis-pituitary gland axis. Pubertal male rats at 35 days of age were maintained on a control diet (Con), whole soybean (SBM) or control diet supplemented with genistein and daidzein (G+D) for 14 days. After sacrifice, tissues were processed by western blot procedures to analyze Mϋllerian inhibiting substance (MIS) (A) and inhibin Bβ in testes (B) and FSHβ (C) and LHβ subunit proteins in pituitary glands (D). Proteins were normalized to actin (Actb) (Inhibin Bβ = 45 kDa, Amh = 74 kDa, Fshβ = 21 kDa, Lhβ = 22 kDa, Actb = 43 kDa. *, P < 0.05 vs. control).