Skip to main content
. 2021 Jan 14;2021:8851888. doi: 10.1155/2021/8851888

Table 1.

Number of alleles, optimal amplification conditions, and fragment length of 29 alleles for the laboratory chickens.

Loci Primer sequence (5′-3′) Temperature(°C) Allele range Applicable groups
MCW0029 GTGGACACCCATTTGTACCCTATG 63.8 139-188 Outbred group
CATGCAATTCAGGACCGTGCA
ADL0293 GTAATCTAGAAACCCCATCT 53.9 106-120 Outbred group
ACATACCGCAGTCTTTGTTC
ADL0317 AGTTGGTTTCAGCCATCCAT 58.5 177-219 Outbred group
CCCAGAGCACACTGTCACTG
GCT0016 TCCAAGGTTCTCCAGTTC 52.2 111-148 Outbred group
GGCATAAGGATAGCAACAG
ADL0304 GGGGAGGAACTCTGGAAATG 53.9 138-161 Outbred group
CCTCATGCTTCGTGCTTTTT
LEI0074 GACCTGGTCCTGACATGGGTG 58.5 221-243 Outbred group
GTTTGCTGATTAGCCATCGCG
ADL328 CACCCATAGCTGTGACTTTG 53.9 107-120 Outbred group
AAAACCGGAATGTGTAACTG
GGANTECl GCGGGGCCGTTATCAGAGCA 65.0 139-194 Outbred group
AGTGCAGGGCGCTCCTGGT
LEI094 CAGGATGGCTGTTATGCTTCCA 56.0 176-211 Outbred group
CACAGTGCAGAGTGGTGCGA
MCW0330 TGGACCTCATCAGTCTGACAG 58.5 217-287 Outbred group
AATGTTCTCATAGAGTTCCTGC
LEI0141 CGCATTTGATGCATAACACATG 52.2 221-245 Outbred group
AAGGCAAACTCAGCTGGAACG
MCW0087 ATTTCTGCAGCCAACTTGGAG 58.5 268-289 Outbred group
CTCAGGCAGTTCTCAAGAACA
MCW0347 GCTTCCAGATGAGCTCCATGG 52.0 121-149 Outbred group
CACAGCGCTGCAGCAACTG
ADL176 TTGTGGATTCTGGTGGTAGC 58.5 183-200 Outbred group
TTCTCCCGTAACACTCGTCA
ADL0201 GCTGAGGATTCAGATAAGAC 58.5 111-151 Outbred group
AATGGCYGACGTTTCACAGC
GGNCAMZO GTCACTAGGTTAGCAGCATG 56.0 234 Outbred group
GCTGGATACAGACCTCGATT Haplotype
GGAVIR AGAGATGGTGCACGCAACCT 60.7 86-89 Outbred group
CGAGCACTTTCTGGCAGAGA Haplotype
MCW0063 GGCTCCAAAAGCTTGTTCTTAGCT 53.9 116-146 Outbred group
GAAAACCAGTAAAGCTTCTTAC Haplotype
ADL185 CATGGCAGCTGACTCCAGAT 58.5 116-142 Outbred group
AGCGTTACCTGTTCGTTTGC Haplotype
GGMYC CGAGGCGCTCTGCGAGTTTA 62.4 139-151 Outbred group
TGGGGACCTCTGGCTCTGAC Haplotype
LEI0094 GATCTCACCAGTATGAGCTGC 53.9 250-283 Outbred group
TCTCACACTGTAACACAGTGC Haplotype
GGVITC AGCCATCATTCAGGGCATCT 58.5 86 Outbred group
GATGTCCTGAGTGATGCTCA Haplotype
ADL0292 CCAAATCAGGCAAAACTTCT 58.5 110-136 Outbred group
AAATGGCCTAAGGATGAGGA Haplotype
GGVITIIG GGCAGGTTTCTAATGCCTGA 56.0 186-189 Outbred group
CCCATCGTTTCAACTGTATG Haplotype
ADL166 TGCCAGCCCGTAATCATAGG 58.5 131-154 Outbred group
AAGCACCACGACCCAATCTA Haplotype
MCW0014 AAAATATTGGCTCTAGGAACTGTC 58.5 172-195 Outbred group
ACCGGAAATGAAGGTAAGACTAGC Haplotype
GGCYMA AGCGAGGCGCTCTGCGAGTT 64.6 140-153 Outbred group
GGGCACCTCTGGCTCTGACC Haplotype
MCW0402 ACTGTGCCTAGGACTAGCTG 56.0 141-229 Outbred group
CCTAAGTCTGGGCTCTTCTG Haplotype
STMSGGHU2-1A CTTAATATGTGTGAGGTGGC 53.9 235-238 Haplotype
GTTCTCACAATTGCATTAGC