Table 2.
Number of alleles, optimal amplification conditions, and fragment length of 28 alleles for the laboratory ducks.
| Loci | Primer sequence(5′-3′) | Temperature (°C) | Allele range | Applicable groups |
|---|---|---|---|---|
| CAUD007 | ACTTCTCTTGTAGGCATGTCA | 60.8 | 100-190 | Outbred group |
| CACCTGTTGCTCCTGCTGT | ||||
| CAUD004 | TCCACTTGGTAGACCTTGAG | 60.8 | 234-385 | Outbred group |
| TGGGATTCAGTGAGAAGCCT | ||||
| CAUD023 | CACATTAACTACATTTCGGTCT | 51.4 | 163-234 | Outbred group |
| CAGCCAAAGAGTTCAACAGG | ||||
| CAUD027 | AGAAGGCAGGCAAATCAGAG | 66.0 | 70-180 | Outbred group |
| TCCACTCATAAAAACACCCACA | ||||
| CAUD001 | ACAGCTTCAGCAGACTTAGA | 55.5 | 150-247 | Outbred group |
| GCAGAAAGTGTATTAAGGAAG | ||||
| CAUD031 | AGCATCTGGACTTTTTCTGGA | 51.4 | 107-187 | Outbred group |
| CACCCCAGGCTCTGAGATAA | ||||
| CAUD032 | GAAACCAACTGAAAACGGGC | 58.1 | 96-206 | Outbred group |
| CCTCCTGCGTCCCAATAAG | ||||
| AY314 | CTCATTCCAATTCCTCTGTA | 50.3 | 112-329 | Outbred group |
| CAGCATTATTATTTCAGAAGG | ||||
| CMO211 | GGATGTTGCCCCACATATTT | 55.0 | 112-205 | Outbred group |
| TTGCCTTGTTTATGAGCCATT | ||||
| APH09 | GGATGTTGCCCCACATATTT | 58.0 | 134-190 | Outbred group |
| TTGCCTTGTTTATGAGCCATTA | ||||
| APH11 | GGACCTCAGGAAAATCAGTGTA | 58.5 | 183-185 | Outbred group |
| GCAGGCAGAGCAGGAAATA | ||||
| APL2 | GATTCAACCTTAGCTATCAGTCTCC | 58.5 | 115-125 | Outbred group |
| CGCTCTTGGCAAATGTCC | ||||
| CAUD011 | TGCTATCCACCCAATAAGTG | 50.3 | 145-223 | Outbred group |
| CAAAGTTAGCTGGTATCTGC | ||||
| CAUD006 | ATGGTTCTCTGTAGGCAATC | 63.5 | 183-290 | Outbred group |
| TTCTGCTTGGGCTCTTGGA | Haplotype | |||
| CAUD018 | TTAGACAAATGAGGAAATAGTA | 50.3 | 100-180 | Outbred group |
| GTCCAAACTAAATGCAGGC | Haplotype | |||
| CAUD010 | GGATGTGTTTTTCATTATTGAT | 50.3 | 138-200 | Outbred group |
| AGAGGCATAAATACTCAGTG | Haplotype | |||
| CAUD012 | ATTGCCTTTCAGTGGAGTTTC | 63.5 | 182-286 | Outbred group |
| CGGCTCTAAACACATGAATG | Haplotype | |||
| CAUD014 | CACAACTGACGGCACAAAGT | 58.1 | 136-200 | Outbred group |
| CTGAGTTTTTCCCGCCTCTA | Haplotype | |||
| CAUD034 | TACTGCATATCACTAGAGGA | 55.5 | 160-296 | Outbred group |
| TAGGCATACTCGGGTTTAG | Haplotype | |||
| CAUD035 | GTGCCTAACCCTGATGGATG | 63.5 | 174-282 | Outbred group |
| CTTATCAGATGGGGCTCGGA | Haplotype | |||
| APL579 | ATTAGAGCAGGAGTTAGGAGAC | 55.0 | 116-227 | Outbred group |
| GCAAGAAGTGGCTTTTTTC | Haplotype | |||
| AY258 | ATGTCTGAGTCCTCGGAGC | 58.1 | 89-162 | Outbred group |
| ACAATAGATTCCAGATGCTGAA | Haplotype | |||
| CMO212 | CTCCACTAGAACACAGACATT | 58.0 | 186-272 | Outbred group |
| CATCTTTGGCATTTTGAAG | Haplotype | |||
| CAUD028 | TACACCCAAGTTTATTCTGAG | 55.5 | 152-226 | Outbred group |
| ACTCTCCAGGGCACTAGG | Haplotype | |||
| CAUD026 | ACGTCACATCACCCCACAG | 60.8 | 134-196 | Outbred group |
| CTTTGCCTCTGGTGAGGTTC | Haplotype | |||
| APH18 | TTCTGGCCTGATAGGTATGAG | 58.0 | 178-325 | Haplotype |
| GAATTGGGTGGTTCATACTGT | ||||
| CAUD002 | CTTCGGTGCCTGTCTTAGC | 60.8 | 200-231 | Haplotype |
| AGCTGCCTGGAGAAGGTCT | ||||
| CAUD005 | CTGGGTTTGGTGGAGCATAA | 60.8 | 184-290 | Haplotype |
| TACTGGCTGCTTCATTGCTG |