Table 4.
Number of alleles, optimal amplification conditions, and fragment length of 16 alleles for the outbred colony laboratory pigeons.
| Loci | Primer sequence(5′-3′) | Temperature (°C) | Allele range |
|---|---|---|---|
| UU-Cli02 | TGGGCAAGGTACACTTTTAGGT | 61.0 | 158-170 |
| CTTTATGCTCCCCCTTGAGAT | |||
| UU-Cli06 | TTTGAAAAACATGGATTGTGC | 56.0 | 140-145 |
| AATTTGCAGAGGGTGAGTGG | |||
| PG5 | GTTCTTGGTGTTGCATGGATGC | 59.0 | 262-266 |
| AGTTACGAAATGATTGCCAGAAG | |||
| C26L9(1265223) | CAAAGCTGCTGACGTGAATCAA | 59.0 | 467-472 |
| AGAGACGCTCCATGCAAAAG | |||
| UU-Cli14 | CAGAACGTTTTGTTCTGTTTGG | 58.0 | 265-292 |
| TCTTGCTGCAGTCTTCATCC | |||
| C12L1(532572) | GTTGTTTGGCTGAGTGGACG | 62.0 | 126-136 |
| TCAACCAGGGGAATTGGCAG | |||
| C12L4(906353) | GCTGCTGTCTTCTTCATTGGG | 60.0 | 210-250 |
| TTAAAACCTCCCGTCTCCCTG | |||
| CliμD11 | CCAATCCCAAAGAGGATTAT | 58.0 | 78-98 |
| ACTGTCCTATGGCTGAAGTG | |||
| C26L10(1404758) | GCTGTCAGGTATCAGCCACAA | 59.0 | 211-226 |
| TCAGACCCACGAAAGCTGTAA | |||
| C26L4(568923) | CAACCCCATGTGGGTGAGAC | 63.0 | 357-432 |
| CACCACCACGTGGGACATC | |||
| PG4 | CCCATCTCCTGCCTGATGC | 64.0 | 136-170 |
| CACAGCAGGATGCTGCCTGC | |||
| UU-Cli12 | CGCCAGACTGTATTGTGAGC | 61.0 | 231-265 |
| AGCATGGCTGTTCTTTGAGG | |||
| CliμT47 | ATGTGTGTTTGTGCATGAAG | 56.0 | 183-214 |
| ATGAAAGCCTGTTAGTGGAA | |||
| CliμD32 | GAGCCATTTCAGTGAGTGACA | 60.0 | 136-158 |
| GTTTGCAGGAGCGTGTAGAGAAGT | |||
| UU-Cli07 | GCTGCCTGTTACTACCTGAGC | 61.0 | 277-310 |
| CTGGCCATGAAATGAACTCC | |||
| C26L1(20390) | AGGAGCCTACACTGGGTTTTC | 60.0 | 250-268 |
| TGTAGCTCTGCAATCAGCCT |