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. 2021 Jan 15;2021:6673661. doi: 10.1155/2021/6673661

Figure 7.

Figure 7

Effect of the W196 replacement in the binding of CHCHD4 to hAIFΔ1-101. Calorimetric titrations of (a) WT CTC, (b) W196Y CTC, (c) W196Aox, and (d) W196A CTC with CHCHD4. The upper plots show the thermograms (thermal power as a function of time), whereas the lower plots show the binding isotherm (normalized heats as a function of the CHCHD4/hAIF molar ratio). Measurements were carried out in 50 mM potassium phosphate, pH 7.4, at 25°C. The CTC forms were obtained by premixing hAIF∆1-101ox and NADH at a 1 : 100 ratio. The binding parameters were estimated through nonlinear least-squares regression applying a single-ligand binding model (continuous lines in binding isotherms).