Figure 4.

Concentration of the nitrosative stress products in HepG2. Cell cultures incubated with (first row) phloroglucinol (PHG), palmitate (NAFLD), both PHG and NAFLD, or palmitate with other known antioxidants: N-acetylcysteine (NAFLD+NAC) and alpha-lipoic acid (NA-FLD+ALA); (second row) phloroglucinol (PHG), hydrogen peroxide (H₂O₂), both PHG and H₂O₂, and hydrogen peroxide with other known antioxidants: N-acetylcysteine (H₂O₂+NAC) alpha-lipoic acid (H₂O₂+ALA). (a, c, d, f) The concentration of NADPH oxidase (NOX) and peroxynitrite was elevated significantly in NAFLD and H₂O₂ groups, respectively. (b, e) The concentration of nitric oxide was lower in the NAFLD and H₂O₂ groups.