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. 2020 Nov 4;119(12):2440–2450. doi: 10.1016/j.bpj.2020.10.031

Figure 4.

Figure 4

EIS with supported lipid bilayers and conductance measurements with porespanning bilayers confirm that HNP1 suppresses LL-37 membrane disintegration. (a) Shown are EIS spectra of supported POPC bilayers at different peptide concentrations. An equivalent circuit used for fitting is also shown. (b) Extracted bilayer resistance and capacitance and their conversion into defect area and bilayer thickness were plotted against peptide concentrations. Experiments were repeated twice and their average and the standard deviation are plotted. (c) Shown are current recordings through free standing lipid bilayer with the addition of LL-37 at 8 μM, HNP1 at 8 μM, and LL-37+HNP1 at 1:1 molar ratio with the total peptide concentration of 16 μM at different applied voltages and (d) extracted I-V plot. For each peptide, multiple runs were analyzed and presented in the I-V plot. (e) Shown are conductance measurements of pore-spanning lipid bilayers exposed to HNP1 at 15 μM at 50 mV holding potential and LL-37+HNP1 mixture at 1:1 molar ratio with the total concentration of 26 μM at −125 mV holding potential. To see this figure in color, go online.