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. 2020 Nov 18;119(12):2558–2572. doi: 10.1016/j.bpj.2020.10.043

Figure 4.

Figure 4

Elastomer mechanics regulate fibroblast polarization but not spreading area or FA size. (A) Adhesion of pHDF on FN-coated elastomers, normalized to their adhesion on FN-coated TCPS, as a function of ζ. Each data point corresponds to an independent experiment with n = 3. Data were compared using one-way ANOVA: NSp > 0.05. (B) Confocal microscopy images of fixed pHDF seeded on FN-coated elastomers for 4 h and stained against F-actin using fluorescent phalloidin to highlight overall cell morphology. Scale bars, 50 μm. (C) High-magnification confocal microscopy images of fixed and immunostained pHDF seeded on FN-coated elastomer for 4 h. Scale bars, 20 μm. (D–G) Column plots (left) showing the average values of cell area (D), aspect ratio (E), FA area (F), and FA length (G) of pHDF seeded on FN-coated elastomers as a function of ζ. Each data point corresponds to the mean value of an independent experiment. Histograms (right) show the relative distribution of values of one such independent experiment. FN-coated glass was used as a control. Mean values for the different elastomers in (D–G) were compared using one-way ANOVA: ∗p < 0.05; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001, and NSp > 0.05. To see this figure in color, go online.