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. 2021 Jan 22;11:2089. doi: 10.1038/s41598-020-80708-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Concentration of LDH released into the medium after (a) 24 h, (b) 48 h, (c) 96 h, (d) 120 h, of incubation with PVC (■) and PMMA ( ) NPs. The data for 72 h incubation is given in the supporting information, Figure S10. The values represent as % LDH release with respect to control (untreated cells) and are the mean ± SEM of three experiments. Statistical analyses were done by one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) compared to control. Unexposed cell lysis solution was used as positive control to obtain the maximum LDH release.

Inline graphic — Concentration of LDH released into the medium after (a) 24 h, (b) 48 h, (c) 96 h, (d) 120 h, of incubation with PVC (■) and PMMA ( ) NPs. The data for 72 h incubation is given in the supporting information, Figure S10. The values represent as % LDH release with respect to control (untreated cells) and are the mean ± SEM of three experiments. Statistical analyses were done by one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001) compared to control. Unexposed cell lysis solution was used as positive control to obtain the maximum LDH release.