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. 2021 Jan 22;11:2089. doi: 10.1038/s41598-020-80708-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Time based tracking of PVC NPs (200 µg/mL) exposed to BHK-21 cells. Top to bottom rows: Hoechst stain in DAPI channel, NPs appear as green in FITC channel, overlay of blue and green channels. Images observed at (A1–A3) 1 min, (B1–B3) 5 min, (C1–C3) 10 min, (D1–D3) 30 min, and (E1–E3) 1 h are given to compare the time dependance internalization of PVC particles. All images were recorded using an Olympus FV1000 confocal microscope. The control experiment for all the time points are given in the supporting information, Figure S6.