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. 2021 Jan 22;11:2089. doi: 10.1038/s41598-020-80708-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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BHK-21 cells exposed to PVC (A1–A4) and PMMA (B1–B4) NPs at a concentration of 200 µg/mL for 24 h and latex beads (80 µg/mL). The images represent nucleus stained with Hoechst dye and cells exposed to PVC or PMMA NPs (green emitting, A2, B2) and red fluorescent latex beads (A3, B3). The blue, green and red fluorescent images are overlaid to show the localization of PVC and PMMA NPs and latex beads inside the cell, indicating that PVC and PMMA NPs are entering the cell by endocytosis.