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. 2021 Jan 22;12:520. doi: 10.1038/s41467-020-20848-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Vulcano plot showing differential splicing of exons identified by limma/diffSplice algorithm. Orange rhombi indicated differentially used exon-exon junctions (diffEEJs) with more than 2-fold change and q < 0.1. b Venn diagramme showing the overlap between diffEEJs identified by limma/diffSplice and JunctionSeq algorithms. c Venn diagramme showing overlap of genes with diffUEs identified by limma/diffSplice and JunctionSeq algorithms. d Pie charts showing the classification of diffEEJs and non-differential exon-exon junctions (non-diffEEJs) according to the modes of alternative splicing. Numbers indicate the percentage of splicing events assigned to a particular mode of splicing. Complex splicing means several (two or more) alternative splicing events occurred simultaneously. e Distribution of the splice sites with diffEEJs and non-diffEEJs among the various functional types of exons. P-values were calculated with χ² test. Panels a to e are based on data from Kasumi-1 cells.