Fig. 2. AmiC, AmcA, and the SPOR_RgsS are required for stable inheritance of RgsS to the newly formed cell poles after cell division.

a mVenus-RgsS localization patterns in cells from exponential phase TY cultures. n, total number of analyzed cells. The values represent the mean values of three biological replicates. The standard deviation values and statistical comparison between the strains are shown in Supplementary Table 3. b Time lapse microscopy images of Rm2011 mVenus-rgsS and its amiC mutant derivative, growing on MM-agarose. Merged mVenus fluorescence and phase contrast pictures are shown. Red and black arrowheads point to cells with localization of mVenus-RgsS foci to old cell poles of the daughter and the mother cells, respectively. Time is shown in minutes. Scale bar, 2 µm. The images are representative of three independent cultivations and microscopy analyses. c mVenus-RgsS foci inheritance observed in time-lapse microscopy. D daughter cell, M mother cell. n total number of analyzed cells. The values represent the mean values of three biological replicates. The standard deviation values and statistic comparison between the strains are shown in Supplementary Table 5.