Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 22;12:545. doi: 10.1038/s41467-020-20739-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — Single-molecule tracking of mVenus-RgsS, mVenus-RgsS_1–₈₆₃, and mVenus- RgsS_1–₈₆₃-mCherry molecules in cells from exponential phase TY cultures. Single molecule tracks were collected from cells, originating from three independent biological replicates. Numbers of single molecule tracks analyzed: Rm2011 mVenus-rgsS, n = 7556; Rm2011 mVenus-rgsS amiC, n = 5439; Rm2011 mVenus-rgsS_1-863, n = 2296; Rm2011 mVenus-rgsS_1-863-mCherry, n = 5469. a Relative fraction size of molecule populations with two different normalized diffusion constants, determined by Gaussian-mixture model (GMM) fit. Statistics analysis and numerical data are presented in Supplementary Table 9. b Estimated average residence times (time a molecule stays in a radius of 128.7 nm for more than nine 20 ms intervals), calculated from single molecule tracking data. Data presented as mean values of residence times calculated for each trajectory. Error bars indicate the standard deviation of the mean. Numerical data is presented in Supplementary Table 9. c Probability heatmaps of mVenus-RgsS, mVenus-RgsS_1–₈₆₃, and mVenus-RgsS_1–₈₆₃-mCherry molecules trajectory distribution in a normalized cell. The color code on the left indicates the probability of the molecules to be detected in the given cell area.