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. 2021 Jan 22;4:98. doi: 10.1038/s42003-020-01643-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–g Immunofluorescence staining signals showing the G4 structures in Saccharomyces cerevisiae (Scer), Drosophila melanogaster (Dmel), Danio rerio (Drer), Pelodiscus sinensis (Psin), Gallus gallus (Ggal), Ovis aries (Oari), and Homo sapiens (Hsap). h Quantification statistics of the number of G4 structures per nucleus in Scer, Dmel, Drer, Psin, Ggal, Oari, and Hsap cells. Signal spots in 30 nuclei from three replicates (ten nuclei for each replicate) were counted for each species. Data are the mean ± SEM (n = 30), statistical significances were determined by Student’s t test, *p < 0.05, **p < 0.01, and ***p < 0.001. The scale bars equal 5 μm.