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. 2021 Jan 22;4:98. doi: 10.1038/s42003-020-01643-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a The methylation levels of the cytosines at upstream 2 kb region of all genes (All) and those genes bearing the (G/C)₃L_1–7 motifs in their upstream 2 kb region (G4) in the pig. Bars from the top to the bottom indicate the value of the three quarters, the median and a quarter respectively. ***p < 0.001 by Wilcoxon test. n = 34,333 cytosine sites for the former and 148,769 cytosine sites for the latter. b Plot of the methylation level distribution in the upstream 2 kb regulatory region of genes in the pig. Methylation levels were calculated in 200 bp sliding windows with a 100 bp step. Proportion of unmethylated and methylated cytosines (c) and the methylated cytosines (mCs) with different methylation levels (d) in the upstream 2 kb region of all genes (All) and those genes bearing the (G/C)₃L_1–7 motifs in their upstream 2 kb region (G4) in the silkworm. High mC hypermethylated cytosines (methylation level > 0.6), mid mC moderate methylated cytosines (0.3 < methylation level < 0.6), low mC hypomethylated cytosines (0 < methylation level < 0.3). **p < 0.01 by chi-square’s test (data subjected to this test were shown in Supplementary Data 1). All: the results of all genes; and G4: the results of the genes bearing the (G/C)₃L_1–7 motifs in their upstream 2 kb region.