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. 2021 Jan 22;11:2132. doi: 10.1038/s41598-021-81734-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NO production and neutralisation of LPS-induced NO production using the Griess assay. (A) NO production by HD11 cells stimulated with 20 µg/ml each peptide after 24 h incubation. * means significantly different between the ABD9 treated and untreated groups (medium group) with p < 0.05. (B) Neutralization of LPS-induced NO production of HD11 cells by 20 µg/ml of AMPs post 24 h stimulation. **** means significantly different from the LPS-treated group (p < 0.0001). One-way ANOVA analysis using the Dunnett post-hoc test was used. All data shown are obtained from at least three independent experiments on different days, means ± SD.