Table 3.
Target genes and primer sequences used for quantitative RT-PCR in this study.
| Target gene | Primer name | Primer sequence (5′–3′) | References |
|---|---|---|---|
| β-actin | β-actin-F | CAACACAGTGCTGTCTGGTGGTA | 53 |
| β-actin-R | ATCGTACTCCTGCTTGCTGATCC | ||
| Ch-IL-8 | Ch-IL8-F | CAGCTGCTCTGTCGCAAG | 54 |
| Ch-IL8-R | GTGGTGCATCAGAATTGAGCT | ||
| Ch-IL-1β | Ch-IL1β-R | GTTGGAGCGGGCAGTCAG | 54 |
| Ch-IL1β-F | GGCATCAAGGGCTACAAGC | ||
| MCP-3 | MCP-3F | CTGCTGCTTCTCCTATGTTCAAC | 55 |
| MCP-3R | ACACATATCTCCCTCCCTTTCTTG | ||
| RANTES | RANTES-F | CCCTCTCCATCCTCCTGGTT | 11 |
| RANTES-R | TATCAGCCCCAAACGGAGAT | ||
| Ch-IFN-γ | Ch-IFN-γ-F | CCAAGAAGATGACTTGCCAGA | 56 |
| Ch-IFN-γ-R | ACCTTCTTCACGCCATCAGG | ||
| Ch-IL-12B(p40) | Ch-IL-12B-F | CCGACTGAGATGTTCCTGGA | 56 |
| Ch-IL-12B-R | CCTGCACAGAGATCTTGTC |
Ch-IL Chicken interleukin, MCP-3 monocyte chemotactic protein 3, RANTES regulated on activation, normal T cell expressed and secreted, IFN-γ interferon-gamma.