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. 2021 Jan 22;11:2080. doi: 10.1038/s41598-021-81335-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Robust 3D axonal outgrowth model allows assessment of neurite disrupting compounds. (A) Montage of a complete OrganoPlate containing 40 chips, each showing 3D axonal outgrowth at day 17 of culture visualized by calcein-AM dye. Maximum projection images, the scale bar is 200 µm. (B) Quantification of axonal outgrowth shown in (A), n = 40. Graph shows mean ± SD. (C) Motor neurons (day 17) were exposed to various concentrations of vincristine for 4 days. Cultures were visualized with calcein-AM dye. Maximum projection images, the scale bar is 200 µm. (D) Quantification of axonal outgrowth depicted in (C). Dose–response curve showing the effect of vincristine exposure on axonal outgrowth. Graph shows mean values ± SEM and a 95% confidence interval (dotted lines) around the curve. n = 5. The graphic representations in (B) and (D) were created using GraphPad Prism, version 8.3.1 (https://www.graphpad.com/scientific-software/prism/).