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. 2021 Jan 22;12:549. doi: 10.1038/s41467-020-20832-7

Fig. 4. Hypoxia activates fetuin-A expression in vitro.

Fig. 4

a Depiction of the potential HREs of mouse Ahsg that were used to generate the luciferase reporter gene constructs (1) to (8). Mutated HREs are shown in blue. b Mean ± SEM of luciferase activity in NRK cells individually transfected with the reporter constructs depicted in a, showing the fold-change in light emission between hypoxic and normoxic culture conditions. Each transfection condition is compared to the empty vector control (pGL3). N = independent experiments. Ordinary one-way ANOVA with Dunnett’s multiple comparisons test. c Expression of fetuin-A and vimentin in primary mouse proximal tubular cells (pPTCs) isolated from four different mice cultured under normoxic or hypoxic conditions. Images are representative of two independent Western blots. Uncropped blots in Source Data. d Relative mRNA expression levels of collagens (Col1a1, Col3a1, and Col6a1), α-smooth muscle actin (Acta2), fibronectin (Fn1), and vimentin (Vim) in kidneys from normoxic (white circles) or hypoxic fetuses (gray circles). Data is shown as mean ± SEM. N = fetal kidneys. Unpaired 2-sided t-test (with Welch’s correction for Col1a1 and Fn1). Individual P-values are denoted above the comparison lines. (****P < 0.0001; **P < 0.01). Source data are provided as a Source Data file.