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. 2021 Jan 22;12:549. doi: 10.1038/s41467-020-20832-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b Fetuin-A supplementation (downward pointing triangles) attenuated the hypoxia-induced expression of the fibrotic markers Acta2 (a) or Col3a1 (b) in pPTCs. Wt and Ahsg KO samples are analyzed separately. Unpaired two-tailed t-test with Welch’s correction (only for comparison of normoxic wt and normoxic Ahsg KO samples). c, d BSA supplementation (upward pointing triangles), did not reduce the expression of Acta2 (c) or Col3a1 (d) in pPTCs. e Fetuin-A supplementation also reduced the expression of fibronectin and collagen type I (Col1a1) protein in pPTCs. Images are representative of two Western blots. Uncropped blots in Source Data. f Relative mRNA expression levels of Tgfb1 shown as mean ± SEM were markedly enhanced in kidneys of hypoxic offspring, regardless of genotype. N = hypoxic or normoxic offspring. g TGF-β1 treatment and hypoxia had an additive effect on the phosphorylation of Smad3 in pPTCs. Images are representative of three Western blots. Uncropped blots in Source Data. h Quantification of Smad3 activation shown in g. N = three independent Western blots. Ordinary one-way ANOVA (Fisher’s LSD test). Data were analyzed from N = pPTCs derived from kidneys of wt or Ahsg KO mice and are presented as mean ± SEM (a–d). Ordinary one-way ANOVA with Tukey’s multiple comparisons test (a–d, f). Individual P-values are denoted above the comparison lines (a–d, f, h). (****P < 0.0001; ***P < 0.001; **P < 0.01). Source data are provided as a Source Data file.