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. 2021 Jan 22;4:104. doi: 10.1038/s42003-020-01623-8

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© The Author(s) 2021, corrected publication 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — The heatmaps show the expression of genes involved in A RNAi, B Imd, C Toll and D Jak-STAT pathways across the developmental stages of ovaries (Ov2, Ov3, Ov4, Ov5, Ove) and in venom glands (vg). The trees on the left are unsupervised hierarchical clustering of expression values. Boxplots represent overall expression of each pathway in ovaries and venom glands. Bold names highlight the genes that are differentially expressed between two stages and dots represent the four different comparisons studied between consecutive ovary stages (Ov2 vs. Ov3, Ov3 vs. Ov4, Ov4 vs. Ov5 and Ov5 vs. Ove). Black, red, and green dots indicate similar, increased and reduced expressions between consecutive developmental stages, respectively. Note that no particular trend appears correlated to bracovirus particles production, which occurs massively from Ov5 onward. The expression of four stable wasp genes having high (RPS18, RPL3, GAPDH) or low expression level (EF1-alpha) is presented as control for comparison.