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. 2021 Jan 22;12(1):118. doi: 10.1038/s41419-021-03398-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Total cellular extracts from CSN6-knockdown and UBR5-knockdown CSN6-knockdown A375 and MV3 cells were prepared and subjected to western blotting with the indicated antibody. B The migration and invasion of CSN6-knockdown and UBR5-knockdown CSN6-knockdown A375 and MV3 cells were quantified. C The growth curves of CSN6-knockdown and UBR5-knockdown CSN6-knockdown A375 and MV3 cells are shown (n = 4). D An in vitro colony formation assay was performed with CSN6-knockdown and UBR5-knockdown CSN6-knockdown A375 and MV3 cells. E, F Stably transfected CSN6-knockdown or UBR5-knockdown CSN6-knockdown A375 cells (1 × 10⁶ cells) or control cells were subcutaneously injected into right dorsal side of female nude mice (n = 6). Tumor volume was measured when the tumors reached a certain volume. Two weeks after injection, the mice were sacrificed, and the tumors were collected and weighed. One-way ANOVA. G Immunohistochemical analysis was conducted to detect CSN6, UBR5, CDK9 and Ki-67 expression, Scale bar = 100 μm. H The model of the CSN6-UBR5-CDK9 axis is shown. All data are shown as the mean ± SD, *P < 0.05, **P < 0.01, and ***P < 0.001. All P-values are based on control versus treatment comparisons.