Fig. 3.

Comparison of RT-qPCR and mNGS derived virus to background ratios. (a) $\stackrel{\sim }{p}$ were determined by mNGS analysis (proportion of virus reads to total reads) and absolute quantification by RT-qPCR (proportion of virus RNA in ng/µl to the total RNA concentration); r = 0.82, **p < 0.0001. (b) Discrepancy of mNGS and RT-qPCR derived $\stackrel{\sim }{p}$ displayed as the factor of $\stackrel{\sim }{p}$_mNGS / $\stackrel{\sim }{p}$_RT-qPCR; median = 61.7.