Table 1.
Sample and sequencing information.
| Viral target | Library ID | Host | Tissue | Total RNA | RT-qPCR | mNGS |
||
|---|---|---|---|---|---|---|---|---|
| (ng/µl) | (Cq value) | total reads | target virus reads | target virus percentage | ||||
| BoDV-1 | lib02012 | Human | brain | 25.7 | 15.7 | 2.69E + 06 | 2.15E + 04 | 8.00E – 01 |
| lib02246 | Human | brain | 37.6 | 23.0 | 7.65E + 06 | 3.20E + 01 | 4.18E – 04 | |
| lib02462 | Human | brain | 17.0 | 17.8 | 4.60E + 06 | 4.61E + 03 | 1.00E – 01 | |
| lib02557 | Human | brain | 4.1 | 19.3 | 1.15E + 07 | 1.96E + 04 | 1.71E – 01 | |
| lib02558 | Human | brain | 18.4 | 20.5 | 3.93E + 06 | 2.70E + 01 | 6.86E – 04 | |
| PGV | lib03148 | European hamster | lung | 136.4 | 26.8 | 1.45E + 06 | 2.10E + 01 | 1.44E – 03 |
| lib03150 | European hamster | lung | 260.2 | 28.0 | 1.76E + 06 | 1.00E + 01 | 5.67E – 04 | |
| RusV | lib03123 | Donkey | brain | 249.0 | 26.2 | 2.65E + 06 | 1.30E + 01 | 4.91E – 04 |
| WNV | lib02898 | Great Grey Owl | organ pool | 598.3 | 11.3 | 6.82E + 06 | 3.97E + 05 | 5.83E + 00 |
| lib02914 | Goshawk | brain | 197.3 | 15.7 | 6.67E + 06 | 3.01E + 04 | 4.52E – 01 | |
| lib02959 | Goshawk | brain | 80.3 | 17.6 | 7.99E + 06 | 2.79E + 04 | 3.49E – 01 | |
| lib03378 | Snowy Owl | heart | 217.4 | 21.4 | 2.71E + 06 | 8.82E + 02 | 3.26E – 02 | |
| lib03379 | Great Grey Owl | liver | 898.5 | 14.3 | 3.00E + 06 | 3.61E + 04 | 1.20E + 00 | |
| lib03380 | Snowy Owl | liver | 832.1 | 12.3 | 3.87E + 06 | 2.12E + 05 | 5.46E + 00 | |
| lib03381 | Blue Tit | brain | 40.6 | 21.7 | 4.38E + 06 | 2.88E + 04 | 6.57E – 01 | |
| lib03382 | Snowy Owl | liver | 715.2 | 16.6 | 3.55E + 06 | 1.84E + 04 | 5.17E – 01 | |
| lib03415 | Snowy Owl | heart | 189.9 | 16.8 | 2.47E + 06 | 9.73E + 03 | 3.94E – 01 | |
| lib03416 | Andean Flamingo | heart | 119.5 | 17.2 | 8.37E + 05 | 3.36E + 03 | 4.02E – 01 | |
| lib03417 | Goshawk | heart | 188.9 | 17.6 | 1.05E + 06 | 3.58E + 03 | 3.41E – 01 | |
| lib03418 | Goshawk | brain | 134.7 | 12.5 | 2.72E + 06 | 2.94E + 05 | 1.08E + 01 | |
| lib03419 | Goshawk | brain | 535.9 | 13.0 | 9.25E + 05 | 4.04E + 04 | 4.37E + 00 | |
| lib03420 | Goshawk | brain | 411.5 | 16.1 | 7.98E + 05 | 5.53E + 03 | 6.93E – 01 | |
| lib03422 | Great Tit | liver/heart | 1180.9 | 11.7 | 1.23E + 06 | 1.56E + 05 | 1.27E + 01 | |
| lib03423 | Eurasian Golden Plover | liver/spleen | 472.7 | 19.2 | 1.58E + 06 | 1.32E + 04 | 8.38E – 01 | |
| lib03424 | Goshawk | brain | 446.0 | 16.7 | 1.02E + 06 | 4.92E + 03 | 4.81E – 01 | |
| lib03425 | Snowy Owl | liver | 619.9 | 14.2 | 1.57E + 06 | 3.21E + 04 | 2.05E + 00 | |
| lib03426 | Snowy Owl | liver | 513.1 | 17.2 | 3.77E + 06 | 1.17E + 04 | 3.10E – 01 | |
| lib03449 | Humboldt-Penguin | heart | 270.7 | 12.1 | 2.72E + 06 | 3.06E + 05 | 1.12E + 01 | |
| lib03450 | Goshawk | brain | 291.5 | 14.5 | 2.32E + 06 | 3.15E + 04 | 1.36E + 00 | |
| lib03451 | Horse | spinal cord | 34.7 | 28.2 | 2.94E + 06 | 1.80E + 01 | 6.11E – 04 | |
Abbreviations: BoDV-1, Borna disease virus 1; PGV, Pegivirus, RusV, Rustrela virus; WNV, West Nile virus lineage 2; RNA (total), ribonucleic acid concentration of the sample; RT-qPCR, reverse transcriptase real-time PCR; Cq, quantification cycle; mNGS, metagenomics next generation sequencing.