Skip to main content
. 2021 Jan 22;12:522. doi: 10.1038/s41467-020-20844-3

Fig. 7. Partial knockout (pKO) of Bclaf1 abrogates the exacerbating effects of lncCIRBIL knockout on cardiac ischemia–reperfusion injury.

Fig. 7

A Effects of Bclaf1 partial knockout on cardiac function of lncCIRBIL knockout mice subjected to ischemia/reperfusion injury. WT-I/R N = 12, CIRBIL-KO-I/R N = 13, CIRBIL-KO-Bclaf1-pKO-I/R N = 17, CIRBIL-KO-Bclaf1-NC-I/R N = 10. *P < 0.05 versus WT-IR, #P < 0.05 versus lncCIRBIL-KO-IR. P values were determined by one-way ANOVA test followed by Bonferroni post hoc analysis. B Infarct size measured by TTC staining (scale bar: 2 mm). WT-I/R N = 6, CIRBIL-KO-I/R N = 7, CIRBIL-KO-Bclaf1-pKO-I/R N = 10, CIRBIL-KO-Bclaf1-NC-I/R N = 7. *P < 0.05 versus WT-IR, #P < 0.05 versus lncCIRBIL-KO-IR. P values were determined by one-way ANOVA test followed by Bonferroni post hoc analysis. C, D Caspase-3 activity and cleaved caspase-3 protein level. N = 8 mice per group for caspase 3 activity, and N = 3 for cleaved caspase-3 protein level. *P < 0.05 versus WT-IR, #P < 0.05 versus lncCIRBIL-KO-IR. P values were determined by one-way ANOVA test followed by Bonferroni post hoc analysis. E Cardiomyocyte apoptosis by TUNEL assay (scale bar: 20 μm). N = 7 mice per group. *P < 0.05 versus WT-IR, #P < 0.05 versus lncCIRBIL-KO-IR. P values were determined by one-way ANOVA test followed by Bonferroni post hoc analysis. F The protein levels of p53 and Bax by western blot. N = 3 mice per group. *P < 0.05 versus WT-IR, #P < 0.05 versus lncCIRBIL-KO-IR. P values were determined by one-way ANOVA test followed by Bonferroni post hoc analysis. Data are expressed as mean ± SEM.