Fig. 4.
The mutation in ZP3 affects interactions between ZP3 and ZP2. a MYC-ZP3 (WT) or MYC-ZP3S173C (MUT) and FLAG-ZP1 co-transfected into HEK293T cells, and analyzed by Co-IP assays. Lanes 1 and 2 show co-immunoprecipitation by anti-MYC antibody, and Lanes 3 and 4 show input. b MYC-ZP3 (WT) or MYC-ZP3S173C (MUT) and GFP-ZP2 co-transfected into HEK293T cells and analyzed by Co-IP assays. Lanes 1 and 2 show co-immunoprecipitation by anti-MYC antibody, and Lanes 3 and 4 show input. Data are expressed as means ± SEM of more than 3 independent experiments. Single asterisk represents P < 0.05