Table 1.

Selection of Samples for phyloscanner Analysis

Analysis Type	No. of Couples Included in Each phyloscanner Run	Samples Included in Each phyloscanner Run	Comments
Sample pair analysis	1 couple	1 sample per person	This approach required knowledge of the relationship between study participants (index–partner); a separate phyloscanner run was performed for each of the 105 possible sample pairs
Group analysis	All 32 couples	1 sample per person	Samples were selected based on timing of sample collection; 4 phyloscanner runs were performed, with a separate run for each set of sample pairs (early/early, late/late, early/late, late/early); this approach provided data for all 105 possible sample pairs and did not require knowledge of the relationship between study participants
Multisample group analysis	All 32 couples	All available samples (1–2 samples/person)	This approach was the same as group analysis, but all data were included in a single phyloscanner run (data from 1–2 samples for each individual); this analysis did not provide data for individual sample pairs

This table describes the approach used to select samples for each type of analysis (sample pair analysis; group analysis; multi-sample group analysis).