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. 2020 Dec 4;29(22):3662–3678. doi: 10.1093/hmg/ddaa258

Figure 4.

Figure 4

Brains of Wbp11+/− embryos are smaller than Wbp11+/+ littermates. (A) Head of Wbp11+/+ (top) and Wbp11+/− (bottom) E17.5 embryos in sagittal section of contrast-enhanced micro-CT scans. (B) Quantification of the total brain volume normalized to each embryo’s weight (P < 0.0001). (C) Schematic diagram of measurements taken from the brains (E17.5). Anteroposterior (AP) length is the maximum length in the AP axis of the brain. Cortical width is the maximum cross-brain distance at the level of lateral ventricle and third ventricle, while cortical thickness is measured at the level of the third ventricle. (D–F) Comparison of Wbp11+/+ to Wbp11+/− measurements of the brain including AP length (D, **P = 0.004), cortical width (E, ****P < 0.0001) and cortical thickness (F, ****P < 0.0001). Embryos included in this data are from the second backcross. Unpaired t-test was used to determine statistical significance. Error bars represent standard deviation (SD). Open dots indicate the embryos in (A). Scale bar: 2 mm (A).