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. 2020 Nov 24;29(22):3631–3645. doi: 10.1093/hmg/ddaa244

Figure 2.

Figure 2

Viability and ATP content of MEFs bearing the indicated OPA1 mutations incubated with ORMs. (A–C) Number of viable cells of ISO1, R445H and D603H MEFs, after incubation for 24 h in DMEM-galactose in the absence or presence of ORMs. Data are expressed as percent of the SRB absorbance measured in DMEM-glucose at time = 0, considered as 100% value. Data are means ± SEM of 19 experiments in DMEM-galactose and 3–7 experiments in DMEM-galactose with ORMs treatment (ORMs 0, 2, 12: n = 3; ORM14, A: n = 7). (D–F) Cellular ATP levels of ISO1, R445H and D603H MEFs after incubation for 24 h in DMEM-galactose ± ORMs. Data are expressed as percentage of ATP content measured in DMEM-glucose at time = 0. Values are means ± SEM of 14 experiments in DMEM-galactose and 3–4 experiments in DMEM-galactose + ORMs (ORMs 0, 2, 12, 14 n = 4, ORMA n = 3). *Denotes P < 0.05.