Figure 6.

Levels of OXPHOS proteins of MEFs bearing the indicated OPA1 mutations incubated with ORMs. MEFs were incubated for 24 h in DMEM-galactose in the absence or presence of ORMs. (A) Western blot of representative subunits of complex I (NDUFA9), complex II (SDHA), complex III (UQCRC2), complex IV (COXIV) and complex V (ATP5A). ACTIN was used as a loading control. (B–F) Densitometric analysis of the OXPHOS subunits levels. Values are means ± SEM (n = 3–9) and are normalized to those of untreated cells in DMEM-galactose. ^*P < 0.05, one simple t test.