FIGURE 4.

The proposed method of action for the use of PBMCsec in flap surgery is shown. (a) The intraoperative application of PBMCsec leads to a reduced rate of flap necrosis and seroma. In the tissue biopsies taken from the border between viable and necrotic tissue, increased rates of angiogenesis were found. (b) The irradiation of PBMC causes a switch toward a regenerative phenotype and induces the secretion of proteins, lipids, and EVs, which form the secretome. (c) PBMCsec resembles the physiologic environment of wound healing and regeneration and therefore has pleiotropic effects